Cloned (Comment) | Organism |
---|---|
gene NAA10, recombinant expression of the enzyme in human 293T cells using a lentiviral vector, recombinant expression of GST-tagged wild-type and mutant Naa10 enzymes in Escherichia coli strain BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockdown of Naa10p by shRNAs, knockdown efficiencies, overview. Generation of truncated Naa10p mutants | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P41227 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant Naa10 enzymes from Escherichia coli strain BL21 by glutathione affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
143-B cell | - |
Homo sapiens | - |
bone | - |
Homo sapiens | - |
HOS cell | - |
Homo sapiens | - |
MG-63 cell | - |
Homo sapiens | - |
MNNG/HOS cell | - |
Homo sapiens | - |
additional information | enzyme levels in different osteosarcoma cell lines, overview | Homo sapiens | - |
osteoblast | located around new bone surfaces | Homo sapiens | - |
osteosarcoma cell | higher NAA10 transcripts in metastatic osteosarcoma tissues compared to non-metastatic tissues. MMP-2 expression levels are also significantly correlated with Naa10p levels in osteosarcoma tissues | Homo sapiens | - |
SAOS-2 cell | - |
Homo sapiens | - |
U2-OS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + N-terminal L-methionyl-[MMP2] | matrix metalloproteinase-2, MMP2, with sequence MEALMAR | Homo sapiens | CoA + H+ + N-terminal Nalpha-acetyl-L-methionyl-[MMP2] | - |
? | |
additional information | no activity with an MMP2 mutated at the acetylytion site of Naa10 | Homo sapiens | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
N-alpha-acetyltransferase 10 | - |
Homo sapiens |
NAA10 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown and overexpression of Naa10p in osteosarcoma cells respectively leads to decreased and increased cell migratory/invasive abilities. Re-expression of Naa10p, but not of an enzymatically inactive mutant, relieves suppression of the invasive ability in vitro and metastasis in vivo imposed by Naa10p-knockdown. The matrix metalloproteinase (MMP)-2 is responsible for the Naa10p-induced invasive phenotype | Homo sapiens |
physiological function | N-alpha-acetyltransferase 10 protein (Naa10p) mediates N-terminal acetylation of nascent proteins. It promotes metastasis by stabilizing matrix metalloproteinase-2 protein in human osteosarcomas via its N-terminal acetylation activity. Oncogenic role of Naa10p, overview. Higher NAA10 transcripts are observed in metastatic osteosarcoma tissues compared to non-metastatic tissues and are also correlated with a worse prognosis of patients. Naa10p is directly associated with MMP-2 protein through its acetyltransferase domain and maintains MMP-2 protein stability via NatA complex activity. MMP-2 expression levels are also significantly correlated with Naa10p levels in osteosarcoma tissues. Function of Naa10p in the regulation of cell invasiveness by preventing MMP-2 protein degradation that is crucial during osteosarcoma metastasis. Naa10p promotes migratory/invasive abilities of osteosarcoma cells, it regulates cell invasion of the osteosarcoma cell lines | Homo sapiens |